Gene editing techniques have witnessed significant traction since CRISPR / CaS9 was discovered. The editing technology has complexities and challenges like the need for palindromic sequence before & after the gene of interest, purification, and expression of CaS nuclease, delivery of gRNA, acting in a non-specific manner resulting in off-shoot mutations and cell toxicity. Despite these limitations, there was a multi-fold interest in the research community to capitalize CRISPR/ CaS9 tool in editing targeted gene sequences.
In recent times, technologies such as TALEN, ZFN, NgAgO, Lambda Red had emerged as novel gene-editing tools having wider applicability with . . .
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